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| Registros recuperados: 225 | |
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| Morga, Benjamin; Faury, Nicole; Guesdon, Stephane; Chollet, Bruno; Renault, Tristan. |
| Since 2008, mass mortality outbreaks associated with the detection of particular variants of OsHV-1 have been reported in Crassostrea gigas spat and juveniles in several countries. Recent studies have reported information on viral replication during experimental infection. Viral DNA and RNA were also detected in the haemolymph and haemocytes suggesting that the virus could circulate through the circulatory system. However, it is unknown if the virus is free in the haemolymph, passively associated at the surface of haemocytes, or able to infect and replicate inside these cells inducing (or not) virion production. In the present study, we collected haemocytes from the haemolymphatic sinus of the adductor muscle of healthy C. gigas spat and exposed them in... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00399/50997/51780.pdf |
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| Renault, Tristan; Stokes, Nancy; Chollet, Bruno; Cochennec, Nathalie; Berthe, Franck; Gerard, Andre; Burreson, Eugene. |
| Two cases of haplosporidian infection occurred during 1993 in Pacific oysters Crassostrea gigas from the French Atlantic coast. The localization and ultrastructure of the plasmodia are described. In situ hybridization of infected tissue sections was conducted with DNA probes for oyster-infecting haplosporidians. The Haplosporidium nelsoni-specific DNA probe MSX1347 hybridized with the C. gigas parasite, and the H, costale-specific probe SSO1318 did not hybridize. Total genomic DNA was extracted from the infected tissue sections for polymerase chain reaction (PCR) amplification of the haplosporidian. PCR amplifications with H. nelsoni-specific primers and with 'universal' actin primers did not yield the expected products of 573 and 700 bp, respectively. A... |
| Tipo: Text |
Palavras-chave: Haplosporidium nelsoni; Haplosporidiosis; Crassostrea gigas; Pacific oyster. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-821.pdf |
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| Renault, Tristan. |
| Les travaux concernant le virus de type herpès, observé chez les huîtres, ont été poursuivis en 1995-1996 et ont ainsi permis d'aboutir à la mise au point d'un protocole de purification du virus, malgré l'ensemble des difficultés rencontrées (impossibilité de reproduire le virus sur lignées cellulaires, dépendance vis à vis des professionnels pour l'approvisionnement en matériel infecté et difficulté de planifier les expériences). De ce fait, le point de blocage que représentait l'obtention de particules virales en nombre suffisant pour le développement d'outils de diagnostic fiables et sensibles a été levé. |
| Tipo: Text |
Palavras-chave: Herpès virus; Pathologie; Virus; Mortalité; Huître creuse; Crassostrea gigas. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00050/16153/13636.pdf |
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| Katarzyna, S; Renault, Tristan; Wolowicz, M. |
| This study presents results of histopathologic, cytogenetics and flow cytometry analyses performed on Macoma balthica collected from the Gulf of Gdansk (Baltic Sea) in 2003 in order to compare the techniques for diagnosis of neoplasia. The proportion of affected clams gave a crude prevalence of 15.7 %. The four stages of the disease defined by histology and three stages of neoplasia defined by flow cytometry were reported. Stage I defined by flow cytometry corresponded to stages I and II defined by histology. Chromosome analysis did not lead to a staging of neoplastic progression. Both cytogenetics and flow cytometry indicated a difference in the DNA content of non-neoplastic and neoplastic cells. Cytogenetics examination marked that the range of... |
| Tipo: Text |
Palavras-chave: Baltic Sea; Cytofluorymetry; Cytogenetics; Histology; Macoma balthica; Neoplasia. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2921.pdf |
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| Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Renault, Tristan. |
| Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence data is available for O. edulis in public databases. Subtracted cDNA libraries were constructed from the O. edulis haemocytes in order to identify genes involved in host reactions against the parasite and quantitative real time PCR assays were developed to study expression of these genes. In this context, identification of reference genes and study of their... |
| Tipo: Text |
Palavras-chave: Real time PCR; Housekeeping genes; Haemocytes; Ostrea edulis; Bonamia ostreae. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12880/9829.pdf |
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| Picot, Sandy; Faury, Nicole; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan; Morga, Benjamin. |
| The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members... |
| Tipo: Text |
Palavras-chave: Autophagy; Autophagy related; Crassostrea gigas; Data mining; Immunohistochemistry; Real-time PCR; Western blot. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71785/70270.pdf |
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| Han,; Kim, Ji Hyung; Renault, Tristan; Choresca, Casiano, Jr.; Shin, Sang Phil; Jun, Jin Woo; Park, Se Chang. |
| Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA,... |
| Tipo: Text |
Palavras-chave: Cyprinid herpes virus 3; Glycoprotein gene; Koi herpesvirus; Microsatellite; Polymorphism; Phylogenetic tree. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00118/22940/20768.pdf |
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| Thebault, Anne; Robert, Maeva; Renault, Tristan; Dumais, M.; Goulletquer, Philippe. |
| Après le naufrage de l'Erika, la question s'est rapidement posée de l'impact des hydrocarbures sur la santé des coquillages, car la pollution concernait des zones de production conchylicoles. Le suivi zoosanitaire en place dans ces zones permettait d'avoir des données de référence avant impact. Il a été choisi différents sites exposés de référence, qui ont fait l'objet d'analyse en toxicologie et en pathologie. A la différence du cas de l'Arnoco Cadiz, l'effort n'a pas été mis uniquement sur les huîtres mais aussi sur d'autres espèces de coquillages, comme les moules et des fouisseurs, particulièrement exposés comme les coques et les palourdes. La mesure de l'exposition effectuée par la Direction de l'Environnement Littoral (MONERlKA) montre qu'une... |
| Tipo: Text |
Palavras-chave: Pollution; Pathologie; Hydrocarbures; Coquillages; HAP; Histologie; Finistère; Vendée. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/00087/19826/17468.pdf |
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| Lupo, Coralie; Osta-amigo, Axel; Mandard, Yann-vari; Peroz, Carole; Renault, Tristan. |
| Farmers’ vigilance is essential for the detection of epidemics, including potential emerging diseases, in marine shellfish. A field study was conducted to investigate oyster farmers’ reporting practices and behaviour, and to identify factors influencing the reporting process of oyster mortality, with the ultimate aim of improving early detection of unexplained oyster mortality outbreaks. A retrospective case-control study of oyster farmers from Charente-Maritime (France) was designed, based on interviews with 27 non-reporting and 89 reporting farmers, further split into 40 formerly-reporting and 49 currently-reporting farmers. Information about farmer and farm characteristics, farming practices, farm health history and related financial compensation on... |
| Tipo: Text |
Palavras-chave: Case-control study; Ordinal logistic regression; Surveillance; Notification; Shellfish diseases. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00190/30132/28594.pdf |
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| Martenot, Claire; Segarra, Amelie; Baillon, Laury; Faury, Nicole; Houssin, Maryline; Renault, Tristan. |
| Immunohistochemistry (IHC) assays were conducted on paraffin sections from experimentally infected spat and unchallenged spat produced in hatchery to determine the tissue distribution of three viral proteins within the Pacific oyster, Crassostrea gigas. Polyclonal antibodies were produced from recombinant proteins corresponding to two putative membrane proteins and one putative apoptosis inhibitor encoded by ORF 25, 72, and 87, respectively. Results were then compared to those obtained by in situ hybridization performed on the same individuals, and showed a substantial agreement according to Landis and Koch numeric scale. Positive signals were mainly observed in connective tissue of gills, mantle, adductor muscle, heart, digestive gland, labial palps, and... |
| Tipo: Text |
Palavras-chave: OsHV-1; Viral proteins; Crassostrea gigas. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00326/43697/43124.pdf |
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| Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan. |
| Pollutants via run-off into the ocean represent a potential threat to marine organisms, especially bivalves such as oysters living in coastal environments. These organisms filter large volumes of seawater and may accumulate contaminants within their tissues. Pesticide contamination in water could have a direct or indirect toxic action on tissues or cells and could induce alteration of immune system. Bivalve immunity is mainly supported by hemocytes and participates directly by phagocytosis to eliminate pathogens. Some studies have shown that pesticides can reduce immune defences and/or modify genomes in vertebrates and invertebrates. Metaldehyde is used to kill slugs, snails and other terrestrial gastropods. Although metaldehyde has been detected in... |
| Tipo: Text |
Palavras-chave: Immunity; Hemocytes; Metaldehyde; Pacific oyster; Flow cytometry; In vivo; Genetic diversity. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00195/30602/29059.pdf |
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| Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle. |
| Apoptosis is a fundamental feature in the development of many organisms and tissue systems. It is also a mechanism of host defense against environmental stress factors or pathogens by contributing to the elimination of infected cells. Hemocytes play a key role in defense mechanisms in invertebrates and previous studies have shown that physical or chemical stress can increase apoptosis in hemocytes in mollusks. However this phenomenon has rarely been investigated in bivalves especially in the flat oyster Ostrea edulis. The apoptotic response of hemocytes from flat oysters, O. edulis, was investigated after exposure to UV and dexamethasone, two agents known to induce apoptosis in vertebrates. Flow cytometry and microscopy were combined to demonstrate that... |
| Tipo: Text |
Palavras-chave: Apoptosis; Hemocyte(s); Oyster; Ostrea edulis; UV; Flow cytometry; Microscopy. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00270/38160/36295.pdf |
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| Registros recuperados: 225 | |
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